Journal: Journal of Experimental Botany

Article Title: A salt-regulated peptide derived from the CAP superfamily protein negatively regulates salt-stress tolerance in Arabidopsis

doi: 10.1093/jxb/erv263

Figure Lengend Snippet: Identification of AtCAPE1 in Arabidopsis . (A) Deduced amino acid sequence of the PROAtCAPE1 product. The predicted secretion signal peptide is underlined. The CAP domain is shaded in grey. The putative AtCAPE1 peptide is shaded in red. The cleavage site predicted to produce AtCAPE1 is indicated with an arrowhead. The putative cleavage signal motif is double-underlined. (B) LC-MS/MS spectrum of the synthetic AtCAPE1. The y-ion is the C-terminal fragments after peptide bond cleavage while the b-ion is the N-terminal fragments. (C) LC-MS/MS spectrum of the identified AtCAPE1 in Arabidopsis . (D) Schema representing the construct used for constitutive overexpression of enhanced yellow fluorescent protein (eYFP)-tagged PROAtCAPE1. The green box shows the CNYx motif. The putative CAPE is shown in red. The numbers indicate the predicted molecular weight of precursor protein tagged with eYFP (45.7kDa) and the cleaved precursor tagged with eYFP (26.3kDa). (E) Production of the precursor PROAtCAPE1 and the cleaved PROAtCAPE1 in CAPE1ox CNYD and CAPE1ox CNAD transgenic plants, where eYFP was fused to PROAtCAPE1 containing wild type (CNYD) and the mutated (CNAD) junction sequence, respectively. T3 seedlings derived from independent transgenic lines were sampled for western blotting with anti-GFP antibody. Coomassie blue staining was used for protein loading control. The lower panel shows the presence of the T-DNA insertions in the transgenic plants by genomic DNA PCR with the primer pair 35S-F’ and eYFP-R’ shown in (D).

Article Snippet: Agilent Arabidopsis (V4) Gene Expression Microarray 4×44k chips were used in this study.

Techniques: Sequencing, Liquid Chromatography with Mass Spectroscopy, Construct, Over Expression, Molecular Weight, Transgenic Assay, Derivative Assay, Western Blot, Staining

Journal: Microarrays

Article Title: Expression Comparison of Oil Biosynthesis Genes in Oil Palm Mesocarp Tissue Using Custom Array

doi: 10.3390/microarrays3040263

Figure Lengend Snippet: Expression signal comparison of selected fatty acid (FA) and triacylglycerols (TAGs). ( A ) tricarboxylic acid cycle (TCA) and glycolysis ( B ) genes at 16 Weeks After Anthesis (WAA) using different microarray platforms, Rice, Arabidopsis and Oil Palm. Signals were compared using a Mann-Whitney test at p -value < 0.05. FAD 2, Oleate desaturase; PK, Pyruvate kinase; LAC, Long-chain acyl-CoA synthetase; FAT A, Acyl-ACP thioesterase A; KAS I, Ketoacyl-ACP synthase I; KAS III, Ketoacyl-ACP synthase III; KAS II, Ketoacyl-ACP synthase II; SAD, Stearoyl-ACP desaturase; FAT B, Acyl-ACP thioesterase B; CPT, Diacylglycerol cholinephosphotransferase; DGAT 2, Acyl-CoA: diacylglycerol acyltransferase 2; LPCAT, 1-acyl glycerol-3-phosphocholine acyltransferase; KAR, Ketoacyl-ACP reductase; ACC CT-α, Carboxyltransferase α-subunit of acetyl-CoA carboxylase; HAD, hydroxyacyl-ACP dehydrase; WRI1, EAR, Enoyl-ACP reductase; PDAT, Phospholipid:diacylglycerol acyltransferase; GPAT, glycerol-3-phosphate acyltransferase; MAT, Malonyl-CoA:ACP malonyltransferase; LPAAT, Lyso PA acyltransferase; DGAT 1, Acyl-CoA:diacylglycerol acyltransferase 1; PDH (DHLAT), Dihydrolipoamide acetyltransferase; SCS, Succinyl coenzyme A synthetase; PGK, Phosphoglycerate kinase; IDH, Isocitrate dehydrogenase; PFK-1, Phosphofructokinase 1; CS, Citrate synthase; PGI, phosphoglucose isomerase; ALDOA, Fructose-bisphosphate aldolase; TPI, Triosephosphate isomerase; ENO 1, enolase 1; ME, Malic Enzyme; ACLY, ATP Citrate Lyase; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; MDH, Malate dehydrogenase; FH, Fumarase; HK, Hexokinase.

Article Snippet: In the cross-species study, the 4 × 44K Arabidopsis (V4) Gene Expression Microarray (G2519F-021169) and the 4 × 44K rice gene expression microarray (G2519F-015241) from Agilent Technologies were used for hybridization with the RNA from the 16 WAA samples.

Techniques: Expressing, Microarray, MANN-WHITNEY

Journal: Microarrays

Article Title: Expression Comparison of Oil Biosynthesis Genes in Oil Palm Mesocarp Tissue Using Custom Array

doi: 10.3390/microarrays3040263

Figure Lengend Snippet: Expression change comparisons of selected FA genes between microarray and Bourgis et al. throughout mesocarp development.

Article Snippet: In the cross-species study, the 4 × 44K Arabidopsis (V4) Gene Expression Microarray (G2519F-021169) and the 4 × 44K rice gene expression microarray (G2519F-015241) from Agilent Technologies were used for hybridization with the RNA from the 16 WAA samples.

Techniques: Expressing, Microarray

Journal: Microarrays

Article Title: Expression Comparison of Oil Biosynthesis Genes in Oil Palm Mesocarp Tissue Using Custom Array

doi: 10.3390/microarrays3040263

Figure Lengend Snippet: Coefficient of correlation of transcriptome sequencing and microarray data for FA genes at 16 WAA. R 2 = 0.569, p -value < 0.01.

Article Snippet: In the cross-species study, the 4 × 44K Arabidopsis (V4) Gene Expression Microarray (G2519F-021169) and the 4 × 44K rice gene expression microarray (G2519F-015241) from Agilent Technologies were used for hybridization with the RNA from the 16 WAA samples.

Techniques: Sequencing, Microarray

Journal: Microarrays

Article Title: Expression Comparison of Oil Biosynthesis Genes in Oil Palm Mesocarp Tissue Using Custom Array

doi: 10.3390/microarrays3040263

Figure Lengend Snippet: Pearson correlation of expression changes between microarray and published data [ 4 ]. * Significant at p -value < 0.05.

Article Snippet: In the cross-species study, the 4 × 44K Arabidopsis (V4) Gene Expression Microarray (G2519F-021169) and the 4 × 44K rice gene expression microarray (G2519F-015241) from Agilent Technologies were used for hybridization with the RNA from the 16 WAA samples.

Techniques: Expressing, Microarray

Journal: Microarrays

Article Title: Expression Comparison of Oil Biosynthesis Genes in Oil Palm Mesocarp Tissue Using Custom Array

doi: 10.3390/microarrays3040263

Figure Lengend Snippet: Expression comparisons of WRI1 between Bourgis et al. ( A ), Tranbarger et al . ( B ) and oil palm microarray ( C ).

Article Snippet: In the cross-species study, the 4 × 44K Arabidopsis (V4) Gene Expression Microarray (G2519F-021169) and the 4 × 44K rice gene expression microarray (G2519F-015241) from Agilent Technologies were used for hybridization with the RNA from the 16 WAA samples.

Techniques: Expressing, Microarray

Journal: Microarrays

Article Title: Expression Comparison of Oil Biosynthesis Genes in Oil Palm Mesocarp Tissue Using Custom Array

doi: 10.3390/microarrays3040263

Figure Lengend Snippet: Expression trend comparison between microarray and qPCR of selected gene candidates throughout mesocarp development.

Article Snippet: In the cross-species study, the 4 × 44K Arabidopsis (V4) Gene Expression Microarray (G2519F-021169) and the 4 × 44K rice gene expression microarray (G2519F-015241) from Agilent Technologies were used for hybridization with the RNA from the 16 WAA samples.

Techniques: Expressing, Microarray